Coding

Part:BBa_K3819062:Design

Designed by: Liyan Wu   Group: iGEM21_UESTC-China   (2021-09-25)


XynB (Xylanase, Aspergillus niger)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized to improve expression in yeast and the restriction site optimized to connect a linker directly.


Source

Aspergillus niger, sequence found through https://pubmed.ncbi.nlm.nih.gov/24888408/

References

Gao H, Yan P, Zhang B, Shan A. Expression of Aspergillus niger IA-001 Endo-β-1,4-xylanase in Pichia pastoris and analysis of the enzymic characterization. Appl Biochem Biotechnol. 2014 Aug;173(8):2028-41. doi: 10.1007/s12010-014-1000-5. Epub 2014 Jun 3. PMID: 24888408.